醛固酮诱导的心肌重构及非洛地平的干预作用

孙建琦; 马礼坤; 余华; 冯克福

醛固酮诱导的心肌重构及非洛地平的干预作用

Felodipine attenuates myocardial remodeling induced by aldosterone

摘要

摘要: 目的研究非洛地平（felodipine，Fel）在醛固酮（aldosterone，Ald）诱导的心肌重构中的保护作用及其可能的机制。方法 SD大鼠32只随机分为4组（每组n=8），即对照组、Ald组、Ald+Fel组和Ald+螺内酯（spironolactone，Spi）组。Ald组：以Ald 18 μg/d皮下注射,连续4周；对照组：注射等量生理盐水4周；Ald+Fel组：以Ald 18 μg/d皮下注射,同时给于Fel 5 mg/(kg·d)灌胃，连续4周；Ald+Spi组:以Ald 18 μg/d皮下注射, 同时给予Spi 20 mg/(kg·d)灌胃，连续4周。比较各组大鼠的左心室质量(LVW)及全心质量(HW)与体质量(BW)的比值，心肌组织匀浆超氧化物歧化酶（SOD）和丙二醛（MDA）的含量，心肌胶原面积（CA），以及心肌细胞凋亡指数（AI）。结果与Ald组相比，对照组、Ald+Fel组和Ald+Spi组大鼠的LVW/BW，HW/BW，CA和AI均减少，对照组和Ald+Spi组大鼠MDA的含量较Ald组减少，SOD的含量较Ald组增加（P<0.01）；Ald+Fel组大鼠SOD和MDA的含量与Ald组相比无明显差异。结论 Fel能够改善Ald诱导的心肌肥大和心肌细胞凋亡，但不能改善Ald诱导的氧化应激水平的增加。

Abstract: AIM To investigated the protective effects of felodipine（Fel） in aldosterone （Ald） induced myocardial remodeling. METHONDS Thirty-two rats were randomly assigned into 4 groups, 8 rats in each group: namely, Control group, Ald group Ald 18 μg/d hypodermic injection for 4 weeks), Ald+Fel group ［Ald 18 μg/d hypodermic injection and Fel 5 mg/(kg·d) intragastric administration for 4 weeks］, and Ald+Spi group (Ald 18 μg/d hypodermic injection and Spi 20 mg/(kg·d) intragastric administration for 4 weeks). All the animals were sacrificed after 4 weeks and the ratio of left ventricular weight/body weight (LVW/BW) and heart weight/body weight (HW/BW), the concentration of SOD and MDA in cardiomyocyte homogenate, collagen area (CA) and apoptosis index (AI) were analyzed. RESUSTS Compared with those in Ald group, LVW/BW, HW/BW, CA and AI were lower in control group, Ald+Fel group and Ald+Spi group. SOD was higher and MDA was lower in control group and Ald+Spi group (P<0.01), but not in Ald+Fel group. CONCLUSION Felodipine can improve aldosterone-induced myocardial hypertrophy and cardiomyocyte apoptosis but not aldosterone-induced oxidative stress.

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