Abstract: AIM:To explore the advantages and disadvantage of three different accesses for central venous catheterization. METHODS: Clinical data of 314 patients who underwent central venous catheterization were retrospectively analyzed. According to different catheterization accesses, 314 cases were divided into three groups: internal jugular vein group (n=103), subclavian vein group (n=105) and femoral vein group (n=106). Success rate of puncture, success rate of central venous catheterization, mean time of catheterization and incidence of different complications were compared among the three groups. RESULTS: Air embolism, deep venous thrombosis or arrhythmia occurred in none of the three groups. Differences among the three groups were not significant in success rate of puncture, success rate of central venous catheterization, mean time of catheterization, incidence of local bleeding, hematoma, hemopneumothorax, central catheterrelated bloodstream infection, catheter occlusion and catheter dislocation. CONCLUSION: The safety and effectiveness of three venous accesses for central venous catheterization showed no significant differences.