Abstract:
AIM To elucidate a possible relationship between TXNIP levels and increased activation of autophagosomes during myocardial ischemia/reperfusion (MI/R) injury.
METHODS Using a MI/R mice model in TXNIP knockout and overexpressed mice, the present investigation observed whether TXNIP levels are linked to autophagy levels and MI/R injury.
RESULTS Compared to Sham mice, the level of TXNIP increased during MI/R (
P<0.01). After MI/R, echocardiography confirmed that TXNIP overexpression aggravated MI/R-induced cardiac dysfunction by lower LVEF% (
P<0.05) and Evansblue/TTC staining revealed that TXNIP overexpression aggravated myocardium infarct size (
P<0.05). In comparison, TXNIP knockout mice showed reduced MI/R-induced cardiac dysfunction by LVEF% (
P<0.05) and infarct size (
P<0.05) compared with wild-type (WT) mice. Further, the present investigation found that elevated TXNIP enhanced MI/R-induced autophagy activation, demonstrated by increased LC3 Ⅱ/I ratio and increased MI/R-induced autophagosome number.
CONCLUSION Elevated TXNIP during MI/R aggravates MI/R-induced cardiac dysfunction, enlarges infarct size, and increases autophagy levels.