Abstract: Weightlessness can lead to change the structure and function of endothelial cells, autophagy and endoplasmic reticulum stress are cells in order to maintain its steady state and response to environmental changes and the stress response, study shows that the simulated weightlessness can improve the standards of vascular endothelial cell proliferation, apoptosis is restrained, cell migration, These physiological changes were accompanied by enhanced autophagy activity of vascular endothelial cells, and increased apoptosis in er stress induced by weightlessness, suggesting that autophagy and ER stress may play a regulatory role in the functional changes of vascular endothelial cells induced by weightlessness. This paper reviews the role of autophagy and endoplasmic reticulum stress in the functional changes of vascular endothelial cells induced by weightlessness.