杜丽, 马玲, 王楠. 红景天苷对高海拔缺氧条件下大鼠心肌自噬相关通路AMPK的影响[J]. 心脏杂志, 2022, 34(4): 378-382. DOI: 10.12125/j.chj.202009073
    引用本文: 杜丽, 马玲, 王楠. 红景天苷对高海拔缺氧条件下大鼠心肌自噬相关通路AMPK的影响[J]. 心脏杂志, 2022, 34(4): 378-382. DOI: 10.12125/j.chj.202009073
    Li DU, Ling MA, Nan WANG. Effects of salidroside on AMPK in myocardial autophagy related pathway under simulated high altitude hypoxia[J]. Chinese Heart Journal, 2022, 34(4): 378-382. DOI: 10.12125/j.chj.202009073
    Citation: Li DU, Ling MA, Nan WANG. Effects of salidroside on AMPK in myocardial autophagy related pathway under simulated high altitude hypoxia[J]. Chinese Heart Journal, 2022, 34(4): 378-382. DOI: 10.12125/j.chj.202009073

    红景天苷对高海拔缺氧条件下大鼠心肌自噬相关通路AMPK的影响

    Effects of salidroside on AMPK in myocardial autophagy related pathway under simulated high altitude hypoxia

    • 摘要:
        目的  探讨红景天苷保护高海拔缺氧损伤大鼠心脏的可能分子机制。
        方法  将40只Wistar大鼠随机分为5组:空白对照组(1 500 m)、缺氧生理盐水组(6 500 m+NS)、缺氧Compound C组(6 500 m+comC)、缺氧红景天苷组(6 500 m +Sal)、缺氧红景天苷+Compound C组(6 500 m +comC+Sal)。造模完成后进行血气分析、HE染色、荧光TUNEL检测及Western blot检测。
        结果  ①缺氧后大鼠心肌结构发生变化,心肌凋亡加重(P<0.05),红景天苷治疗可改善心肌细胞病理性损伤,减少心肌细胞凋亡(P<0.05);②缺氧后磷酸化AMPK水平上升,磷酸化mTOR水平降低(P<0.05)。而Compound C组AMPK及mTOR表现呈相反趋势,红景天苷治疗后AMPK及mTOR的表达水平介于Compound C组与红景天苷组之间,但是自噬蛋白Beclin-1的表达较Compound C组增加(P<0.05)。
        结论  红景天苷可以正向调节AMPK信号通路激活自噬,对缺氧损伤的大鼠心脏起保护作用。

       

      Abstract:
        AIM  To investigate the molecular pharmacological mechanism of Salidroside in protecting rat heart injury induced by high altitude hypoxia.
        METHODS  Wistar rats were randomly divided into 5 groups: blank control group (1 500 m), hypoxic saline group (6 500 m+NS), hypoxia compound C group (6 500 m+comC), hypoxia salidroside group(6 500 m+Sal), hypoxia salidroside+ compound C group (6 500 m+comC+Sal).
        RESULTS  After exposure to hypoxic environment, myocardial structure was changed and myocardial apoptosis was aggravated(P<0.05). Salidroside treatment could improve the pathological damage of myocardial cells and reduce the degree of myocardial apoptosis(P<0.05); At the same time, the level of phosphorylated AMPK increased and the level of phosphorylated mTOR decreased(P<0.05). The expression levels of AMPK and mTOR were between compound C group and salidroside group, but the expression of autophagy protein Beclin-1 was higher than that of compound C group(P<0.05).
        CONCLUSION   salidroside can positively regulate AMPK signaling pathway, activate autophagy and protect myocardium from hypoxia injury.

       

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