江丽青, 李凯峰, 翟蒙恩, 夏麟, 王晓武, 俞世强, 段维勋. 四氢姜黄素对2型糖尿病小鼠糖脂代谢及大血管病变的影响[J]. 心脏杂志, 2020, 32(2): 114-118. DOI: 10.12125/j.chj.201908037
    引用本文: 江丽青, 李凯峰, 翟蒙恩, 夏麟, 王晓武, 俞世强, 段维勋. 四氢姜黄素对2型糖尿病小鼠糖脂代谢及大血管病变的影响[J]. 心脏杂志, 2020, 32(2): 114-118. DOI: 10.12125/j.chj.201908037
    Li-qing JIANG, Kai-feng LI, Meng-en ZHAI, Lin XIA, Xiao-wu WANG, Shi-qiang YU, Wei-xun DUAN. Effects of tetrahydrocurcumin on glucose and lipid metabolism and macrovascular complications in type 2 diabetic mice[J]. Chinese Heart Journal, 2020, 32(2): 114-118. DOI: 10.12125/j.chj.201908037
    Citation: Li-qing JIANG, Kai-feng LI, Meng-en ZHAI, Lin XIA, Xiao-wu WANG, Shi-qiang YU, Wei-xun DUAN. Effects of tetrahydrocurcumin on glucose and lipid metabolism and macrovascular complications in type 2 diabetic mice[J]. Chinese Heart Journal, 2020, 32(2): 114-118. DOI: 10.12125/j.chj.201908037

    四氢姜黄素对2型糖尿病小鼠糖脂代谢及大血管病变的影响

    Effects of tetrahydrocurcumin on glucose and lipid metabolism and macrovascular complications in type 2 diabetic mice

    • 摘要:
        目的  探讨四氢姜黄素(THC)对2型糖尿病(T2MD)小鼠糖脂代谢的影响及其血管的保护作用。
        方法  60只体质量(20~25)g雄性C57BL/6J小鼠,随机取15只小鼠作为正常对照(Con)组,正常饮食,其余小鼠均以高糖脂饮食结合小剂量连续腹腔注射链脲佐菌素(STZ)的方法建立T2MD小鼠模型。造模成功后随机分为模型(T2MD)组和治疗(T2MD+THC)组,治疗组给THC药12周。实验结束后,检测血清总胆固醇(TC)、甘油三酯(TG)、血清葡萄糖(GLU)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C)含量;取小鼠主动脉观察其病理形态学改变,Western blot法检测Notch1信号关键分子NICD、氧化应激标志分子gp91phox、平滑肌表型标志分子α-SMA的表达水平。
        结果  与模型组比较,THC治疗组可以显著改善T2MD小鼠糖脂代谢异常,主动脉组织中NICD表达升高、gp91phox明显降低、α-SMA上调,血管形态得到明显改善(P<0.05)。
        结论  THC可以明显改善T2MD小鼠糖脂代谢异常,并发挥大血管保护作用,其可能机制是否通过Notch1信号通路调节氧化应激损伤进而保护血管有待进一步研究。

       

      Abstract:
        AIM  To investigate the effects of tetrahydrocurcumin (THC) on glucose and lipid metabolism and its role of vascular protection in type 2 diabetic mice.
        METHODS  Sixty male C57BL/6J mice (20~25)g were used in this study. Fifteen mice were randomly selected as a control group and were given a normal diet and a mouse model of type 2 diabetes was established in the rest mice. To establish the model, the mice were fed with high-glycolipid diet for 4 weeks and then they were injected consecutively three times with streptozotocin (STZ) at a dose of 60 mg/kg. After successful establishment of the model, the mice were randomly divided into a model group (T2MD) and a tetrahydrocurcumin group (T2MD+THC), in which T2MD+THC was administered for 12 weeks. After the experiment, the mice were anesthetized and blood was taken from the carotid artery. The serum was separated from the whole blood and then the contents of total cholesterol (TC), triglyceride (TG), serum glucose (GLU), high-density lipoprotein (HDL-C) and low-density lipoprotein were detected. The pathological morphology of the aorta of the mice were observed in each group. The expression levels of NICD (as the key molecule of Notch1 signaling), gp91phox (as the marker molecule of oxidative stress) and α-SMA (as the marker molecule of the smooth muscle phenotype) were detected by Western blot.
        RESULTS  THC treatment significantly improved the abnormal glucose and lipid metabolism in type 2 diabetic mice, up-regulated NICD and α-SMA expressions, down-regulated gp91phox expression (all P < 0.01), and significantly improved vascular morphology.
        CONCLUSION  THC significantly improves abnormal glucose and lipid metabolism in type 2 diabetic mice and protects macrovascular. The possible mechanism appears to be vascular protectioin by regulating oxidative stress damage through the Notch1 signaling pathway.

       

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