肖懿慧, 袁祖贻. 普洱茶提取物对巨噬细胞株RAW264.7凋亡的作用[J]. 心脏杂志, 2019, 31(5): 506-509, 520. DOI: 10.12125/j.chj.201901012
    引用本文: 肖懿慧, 袁祖贻. 普洱茶提取物对巨噬细胞株RAW264.7凋亡的作用[J]. 心脏杂志, 2019, 31(5): 506-509, 520. DOI: 10.12125/j.chj.201901012
    Yi-hui XIAO, Zu-yi YUAN. Pu-erh tea ameliorates atherosclerosis through reducing NF-κB activation in vitro study[J]. Chinese Heart Journal, 2019, 31(5): 506-509, 520. DOI: 10.12125/j.chj.201901012
    Citation: Yi-hui XIAO, Zu-yi YUAN. Pu-erh tea ameliorates atherosclerosis through reducing NF-κB activation in vitro study[J]. Chinese Heart Journal, 2019, 31(5): 506-509, 520. DOI: 10.12125/j.chj.201901012

    普洱茶提取物对巨噬细胞株RAW264.7凋亡的作用

    Pu-erh tea ameliorates atherosclerosis through reducing NF-κB activation in vitro study

    • 摘要:
        目的  通过观察普洱茶提取物对巨噬细胞株RAW264.7凋亡/迁移的作用以及可能的机制,探讨普洱茶改善动脉粥样硬化的可能机制。
        方法  ①分组:普洱茶提取物处理细胞时间分组:0 h、12 h、24 h、36 h、48 h、60 h、72 h;普洱茶提取物浓度分组:0、1/64、1/128、1/256、1/512、1/1024;②TUNEL法检测巨噬细胞凋亡;③实时定量PCR、Western blot检测 P65、IκB-β表达水平;④Transwell实验观察细胞迁移能力。
        结果  ①普洱茶提取物促进巨噬细胞的凋亡,且具有时间及浓度依赖性;②普洱茶提取物上调IκB-β mRNA及蛋白表达,且具有时间及浓度依赖性;普洱茶提取物下调P65 mRNA及蛋白表达,且具有时间及浓度依赖性;③普洱茶提取物抑制巨噬细胞迁移率,Bay11-7085(NF-κB活化抑制剂)同样抑制巨噬细胞迁移;Bay11-7085和普洱茶提取物同时与细胞共培养并不增加对细胞迁移能力的抑制。
        结论  普洱茶提取物通过抑制NF-κB信号通路促进巨噬细胞凋亡并抑制其迁移,可能是其抗动脉粥样硬化的作用机制。

       

      Abstract:
        AIM  To investigate the possible mechanism of Pu-erh tea in reversing atherosclerosis by observing the effects of Pu-erh tea extract on apoptosis/migration, P65/IκB-β gene and protein expression of macrophage RAW264.7.
        METHODS  Grouping: Cells treated with Pu-erh tea extract were divided into groups of 0, 12, 24, 48, 60 and 72 hours; Pu-erh tea extract concentration groups were 0, 1/128, 1/256, 1/512 and 1/1024; TUNEL method was used to detect apoptosis of macrophages; real-time quantitative PCR and Western-blot were used to detect the expression levels of P65 and IκB-β; and Transwell experiment was used to observe cell migration ability.
        RESULTS  ①Pu-erh tea extract promoted apoptosis of macrophages in a time and concentration dependent manner; ②Pu-erh tea extract upregulated the expression of IκB-β in a time and concentration dependent manner; ③ Pu-erh tea extract inhibited the migration rate of macrophages, Bay11-7085 (NF-kappa B activation. Inhibitors) also inhibited macrophage migration; Bay11-7085 and Pu-erh tea extracts co-cultured with cells did not increase the inhibition of cell migration.
        CONCLUSION  Pu-erh tea extract promoted macrophage apoptosis and inhibited its migration through NF-kappa B pathway, which may be the anti-atherosclerosis mechanism of Pu-erh tea extract

       

    /

    返回文章
    返回